Investigating how macromolecules interact with their native cellular environment is needed to fully understand their biological functions, as well as their dysfunction in disease states.  Raman scattering is an excellent tool to probe these interactions since it monitors molecular vibrations that are intrinsically sensitive to the local chemical environment.  Raman spectra directly report, among other things, on the local electric field strengths, dihedral bond angles, and interaction energies of proteins, nucleic acids, and lipids.  Spontaneous Raman scattering, however, is intrinsically weak, making it unsuitable for high-speed live-cell imaging.  Stimulated Raman scattering (SRS) overcomes this problem by using two laser beams to enhance vibrational transitions. As a result, SRS enables video-rate biological imaging with a greater sensitivity than spontaneous Raman scattering.  Despite this, the rich chemical information encoded in Raman spectra has surprisingly not been exploited in SRS imaging applications. Our group is working towards bridging this gap by combining the chemical insights from Raman spectroscopy with advanced SRS imaging techniques to incisively study biophysical problems in living cells and tissue.